CURRICULUM VITAE
Henry V. Huang

PubMed Publications: for huang + HV
Division of Biology and Biomedical Sciences profile

Present Position:

1992 - Associate Professor Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri

Education:

1972	A.B. Biology, Occidental College, Los Angeles, California
1977	Ph.D. Biochemistry, California Institute of Technology, Pasadena, California. Thesis advisor: Dr. William J. Dreyer.

Professional Experience and Appointments:

1982-1992	Assistant Professor Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri
1981-1982	Senior Research Fellow Division of Biology, California Institute of Technology, with Dr. Leroy Hood
1982	Invited Instructor faculty course on techniques in molecular biology, California State University, Northridge, California.
1980-1981	Senior Research Fellow in Biology and Biomedical Engineering Divisions of Biology, and Engineering and Applied Science, California Institute of Technology, with Drs. Leroy Hood and Derek Fender
1979-1980	Research Fellow in Biology and Biomedical Engineering Divisions of Biology, and Engineering and Applied Science, California Institute of Technology, with Drs. Leroy Hood and Derek Fender
1979-1980	Part time Instructor Division of Biology, California Institute of Technology
1977-1979	American Cancer Society Postdoctoral Fellow Division of Biology, California Institute of Technology, with Dr. Leroy Hood
1971-1972	Member of BUGSYS design team for computer analysis of video images Jet Propulsion Laboratory, California Institute of Technology

Honors:

1970	Seltzer Award, Occidental College
1970-1972	College Scholar, Occidental College
1972	A.B. Summa Cum Laude, Occidental College
1972	Alpha Mu Gamma, National Honorary Language Fraternity
1972	Phi Beta Kappa
1972	Association for the Advancement of Biomedical Education Award
1972-1977	Danforth Fellow
1976-1977	McCallum Fellowship, California Institute of Technology
1977-1979	American Cancer Society Postdoctoral Fellow
1995-1999	Member, NIH Virology Study Section

International Committees:

1997-present International Committee on Taxonomy of Viruses. Togaviridae Study Group

Editorial Responsibilities:

1982- Ad Hoc Reviewer: Journal of Virology, Proceedings of the National Academy of Science, USA, Virus Research, Nucleic Acids            Research

Professional Society Memberships:

American Association for the Advancement of Science

American Society for Microbiology

American Society for Virology

Genetics Society of America

Sigma Xi

University Appointments and Committees:

Thesis committees 1982-present
Patricia Ahrweiler Mari Aker* J.D. Alvarez Sean Amberg Marion E. Andrew Charles F. Austerberry Frederick G. Barr Jenny M. Buzan Alejandro Carillo David Davido Anthony M. Dean Karen W. Dodson Stacy Finkbeiner Wadiha Freije Danny Garza Brian Greuel Brooke Harmon Jennifer Hertz* Jeffrey Hiken* William C. Hollifield* Roberta Hopmann Kathryn Kellerman Mark LaStarza Julie Lemm Robin Levis* Guangpu Li Randall Little Thomas M. J. Niederman Hal Padgett Elizabeth Paine J Christian Perez Michael Reagan Regina Rowe Scott R. Schaecher Michael V. Seiden Ping Shen* Lisa Strelow David Sweetser Lai-Wa Tam Ivan Tarle Judy A. C. Thomas Mohammed Tondravi Manuel Tsiang Tony Weisstein Duan-Zhi Wen Kathleen Weston Matthew Wielgosz* Mollie Winfield Ruth W. Yokoyama * Thesis advisor of these students
Other committees
1983-5	Member, Nucleic Acids Preliminary Examination Committee
1986	Chair, Nucleic Acids Preliminary Examination Committee
1985-86, 88	Member, Developmental Biology Preliminary Examination Committee
1987	Chair, Developmental Biology Preliminary Examination Committee
1988	Member, Genetics Preliminary Examination Committees
1991	Chair, Virology Preliminary Examination Committee
1983	Member, Cell and Molecular Biology Programs Admissions Committee
1984-87	Secretary, Cell and Molecular Biology Programs Admissions Committee
1988	Secretary, Cell, Immunology and Molecular Biology Programs Admissions Committee
1988-1992	Member, Medical Scientist Training Program Committee
1991	Medical Scientist Training Program Director Search Committee
1990-present	Supervisor, Departmental computing services
1986-1988	Member, Steering Committee of the Molecular Biology: Genetics and Biochemistry Program
1988-1991	Co-coordinator, Molecular Biology: Genetics and Biochemistry Program
1991-1992	Coordinator, Molecular Microbiology & Microbial Pathogenesis Program
1991-1992	Member, Governing Committee of the Programs in Cell and Molecular Biology
1990-1991	Senior Advisory Committee
1991	Co-chairman, Cell Molecular Biology Merger Committee
1992	Member, Programs and Student Affairs Committee
1992-1994	Member, Minority subcommittee, Committee on Admissions, School of Medicine
1992-1996	Member, Recruitment Committee of the Division of Biology and Biomedical Sciences
1997	Information Services Committee, of the Medical School Process Improvement Program
1996-2000	Member, Committee on Admissions, School of Medicine
2001-2005	Member, Institutional Biological and Chemical Safety Committee
2005-present	Co-chair, Institutional Biological and Chemical Safety Committee
Teaching
1983	Microbiology: Molecular Biology of Prokaryotes
1983, 1984	Foundations in Immunology
1983, 1984	Topics in Immunology
1983, 1986	Regulatory Phenomena in Cell and Molecular Biology
1984	Selected Topics in Developmental Biology: Mechanisms of DNA Recombination and Rearrangement
1987	Advanced Genetics
1989	Practical Applications of Viruses
1990-91	Microbiology
1994	Molecular Virology
1995	Ethics and Research Science
1995-1998	Co-coursemaster, Molecular Virology
1997-present	Molecular Microbiology and Pathogenesis
1999-2004	Coursemaster, Molecular Virology
1999-2001	Microbes and Pathogenesis
2000-2001	Discussion group leader, Nucleic acids and Protein Synthesis
2004	Co-coursemaster, Special Topics in Microbial Pathogenesis
2002-present	Co-coursemaster, Microbes and Pathogenesis
Participation on Training Grants
1984-present	Cell and Molecular Biology Training Grant
1984-present	Program Physician-Scientist Award
1984-present	Infectious Disease Training Grant
Peer Review Panels
1982-present	Ad hoc and special reviewer / site visit panels, for NIADDK, NIAID, NSF.
1995-1999	Member, Virology study section, NIH.
Community Service
1989-1997	Judge, Monsanto-St. Louis Post Dispatch Science Fair, Honors Division.
1994-2000	Technology Committee, Forsyth School
1995-2003	Board of Trustees, Forsyth School

Publications:

1. Huang, H.V., R.S. Molday and W.J. Dreyer (1973). Isoelectric focusing of rod outer segment membrane proteins. FEBS Letters 37: 285-289.
   
2. Lim, R.W., R.S. Molday, H.V. Huang and S.-P.S. Yen (1975). Application of latex microspheres in the isolation of plasma membranes. Affinity density perturbation of erythrocyte membranes. Biochim. Biophys. Acta 394: 377-387.
   
3. Hood, L., H.V. Huang and W.J. Dreyer (1977). The Area Code Hypothesis: The immune system provides clues to understanding the genetic and molecular basis of cell recognition during development. J. Supramolec. Struct. 7: 531-559.
   
4. Huang, H.V. and W.J. Dreyer (1978). Bursectomy in ovo blocks the generation of immunoglobulin diversity. J. Immunol. 121: 1738-1747.
   
5. Hood, J.M., H.V. Huang and L. Hood (1980). A computer simulation of evolutionary forces controlling the size of a multigene family. J. Mol. Evol. 15: 181-197.
   
6. Early, P., H. Huang, M. Davis, K. Calame and L. Hood (1980). An immunoglobulin heavy chain variable region gene is generated from three segments of DNA: VH, D and JH. Cell 19: 981-992.
   
7. Huang, H.V., S.T. Crews and L. Hood (1981). The arrangement, rearrangement and diversification of antibody genes. In Frontiers in Immunogenetics, W. Hildemann and J. Frelinger (eds), Elsevier-North Holland, New York. pp63-74.
   
8. Huang, H.V., S.T. Crews and L. Hood (1981). Diversification of antibody genes through DNA rearrangement. Adv Exp Med Biol 137:475-488.
   
9. Crews, S.T., J. Griffin, H. Huang, K. Calame and L. Hood (1981). A single VH gene segment encodes target="ref" the immune response to phosphocholine: Somatic mutation is correlated with the class of the antibody. Cell 25: 59-66.
   
10. Huang, H.V., S.T. Crews and L. Hood (1981). An immunoglobulin VH pseudogene. J. Appl. Molec. Genet. 1: 93-101.
    
11. Hood, L., M. Davis, P. Early, K. Calame, S. Kim, S. Crews and H. Huang (1981). Two types of DNA rearrangements in immunoglobulin genes. Cold Spring Harbor Symp. Quant. Biol. 45: 887-898.
    
12. Hood, L. J. Griffin, S. Crews, H. Huang, M. Kronenberg and S. Kim (1981). Antibody and MHC genes. In Immunoglobulin Idiotypes and their Expression. C. Janeway, E. Sercarz, H. Wigzell and C. Fred Fox (eds), Academic Press, New York. ICN-UCLA Symp. Molec. Cell. Biol. 20: 805-824.
    
13. Huang, H.V. and L. Hood (1982). The expression of antibody genes. In Comparative Research on Leukemia and Related Diseases. D.S. Yohn and J.R. Blakeslee (eds), Elsevier-North Holland, New York. pp 169-172.
    
14. Hunkapiller, T., H. Huang, L. Hood and J. Campbell (1982). The impact of modern genetics on evolutionary theory. In Perspectives on Evolution. R. Milkman (ed), Sinauer Assoc., Sunderland, Mass. pp 164-189.
    
15. Hood, L., J. Griffin, S. Crews, H. Huang, K. Kronenberg and S. Kim (1982). Antibody and MHC genes. Oncodev Biol. Med. 4: 61-79.
    
16. Huang, H.V., M. Bond, M. Hunkapiller and L. Hood (1983). Efficient cleavage of proteins at tryptophanyl residues using DMSO-HCl and CNBr. Meth. Enzymol. 91: 318-324.
    
17. Huang, H.V. and L. Hood (1983). The ontogenetic and evolutionary origin of V gene formation. Stadler Genetics Symp. Proc. 14: 59-68.
    
18. Huang, H.V. and L. Hood (1983). Origins of antibody diversity. In Recombinant DNA and Medical Genetics. L. Porter (ed). Academic Press, New York. Birth Defects Symp. 13: 97-104.
    
19. Levis, R., B.G. Weiss, M. Tsiang, H. Huang and S. Schlesinger (1986). Deletion mapping of Sindbis virus DI RNAs derived from cDNAs defines the sequences essential for replication and packaging. Cell 44: 137-145.
    
20. Shen, P. and H.V. Huang (1986). Homologous recombination in Escherichia coli: Dependence on substrate length and homology. Genetics 112: 441-457.
    
21. Lutz, C.T., T.L. Bartholow, N.S. Greenspan, R.J. Fulton, W.J. Monafo, R.M. Perlmutter, H.V. Huang and J.M. Davie (1987). Molecular dissection of the murine antibody response to streptococcal group A carbohydrate. J. Exp. Med. 165: 531-545.
    
22. Schlesinger, S., R. Levis, B.G. Weiss, M. Tsiang and H. Huang (1987). Replication and packaging sequences in defective interfering RNAs of Sindbis virus. In Positive Strand RNA Viruses, M. Brinton and R. Rueckert (eds). Alan R. Liss, New York. pp 241-250.
    
23. Siu, G., E. A. Springer, H. V. Huang, L. E. Hood and S. T. Crews (1987). Structure of the T15 VH gene subfamily: Identification of immunoglobulin gene promotor homologies. J. Immunol. 138: 4466-4471.
    
24. Lutz, C.T., W.C. Hollifield, B. Seed, J.M. Davie and H.V. Huang (1987). Syrinx2A: An improved lambda phage vector designed for screening DNA libraries by recombination in vivo. Proc. Natl. Acad. Sci. USA 84: 4379-4383.
    
25. Levis, R., S. Schlesinger and H.V. Huang (1987). Engineered defective interfering RNAs of Sindbis virus express bacterial chloramphenicol acetyltransferase in avian cells. Proc. Natl. Acad. Sci. USA 84: 4811-4815.
    
26. Rice, C.M., R. Levis, J.H. Strauss and H.V. Huang (1987). Production of infectious RNA transcripts from Sindbis virus cDNA clones: Mapping of lethal mutations, rescue of a temperature-sensitive marker, and in vitro mutagenesis to generate defined mutants. J. Virol. 61:3809-3819.
    
27. Hollifield, W.C., E.N. Kaplan and H.V. Huang (1987). Efficient RecABC-dependent, homologous recombination between lambda and plasmids requires a phage ninR region gene. Molec. Gen. Genet.210:248-255.
    
28. Nag, D. K., H. V. Huang and D. E. Berg (1988). Bidirectional chain terminating DNA sequencing strategy using transposon Tn5-seq as a source of mobile primer sites. Gene 64:135-145.
    
29. Polo, J. M., N. L. Davis, C. M. Rice, H. V. Huang and R. E. Johnston. (1988) Molecular analysis of Sindbis virus pathogenesis in neonatal mice using virus recombinants constructed in vitro. J. Virol. 62:2124-2133.
    
30. Huang, H.V., P. Little and B. Seed (1988). Improved suppressor tRNA cloning vectors and plasmid-phage recombination. Biotechnology 10:269-83
    
31. Schlesinger, S., C. Xiong, R. Levis, C. M. Rice and H. V. Huang. (1988) Development of Sindbis virus and defective interfering RNAs as expression vectors. In Viral Vectors, Y. Gluzman and S. H. Hughes (eds). Cold Spring Harbor Banbury Conference Report. Cold Spring Harbor Laboratory, New York. pp 167-171.
    
32. Xiong, C., R. Levis, P. Shen, S. Schlesinger, C. M. Rice and H. V. Huang. (1989) Sindbis virus: an efficient, broad host range vector for gene expression in animal cells. Science 243:1188-1191.
    
33. Phadnis, S., H. V. Huang and D. E. Berg. (1989) Tn5supF, a 264 bp transposon derived from Tn5 for insertion mutagenesis and sequencing DNAs cloned in phage lambda. Proc. Natl. Acad. Sci. USA 86:5908-5912.
    
34. Shen, P. and H.V. Huang. (1989) Effect of base pair mismatches on recombination via the RecBCD pathway. Molec. Gen. Genet. 218:358-360.
    
35. Mi, S., Durbin, R., Huang, H. V., Rice, C. M., and Stollar, V. (1989). Association of the Sindbis virus RNA methyltransferase activity with the nonstructural protein nsP1. Virology. 170:385-391.
    
36. Grakoui, A., R. Levis, R. Raju, H. V. Huang and C. M. Rice. (1989) A cis-acting mutation in the Sindbis virus junction region affecting subgenomic RNA synthesis. J. Virol. 63:5216-5227.
    
37. Huang, H. V., C. M. Rice, C. Xiong and S. Schlesinger. (1989) RNA Viruses as Gene Expression Vectors. Virus Genes 3: 85-91.
    
38. Grakoui, A., R. Levis, R. Raju, S. Schlesinger, H. V. Huang and C. M. Rice. (1990) Cis-acting sequence responsible for Sindbis virus subgenomic RNA synthesis. in New Aspects of Positive-Strand RNA Viruses, M. A. Brinton & F. X. Heinz (ed). American Society of Microbiology, Washington D.C. pp108-115.
    
39. Levis, R., S. Schlesinger and H. V. Huang. (1990) The promoter for Sindbis virus RNA-dependent subgenomic mRNA transcription. J. Virol. 64:1726-1733.
    
40. Raju, R. and H. V. Huang. (1991) Analysis of Sindbis virus promoter recognition in vivo using novel vectors with two subgenomic mRNA promoters. J. Virol. 65:2501-2510.
    
41. Hertz, J. and H. V. Huang. (1992) Utilization of heterologous alphavirus junction sequences as promoters by Sindbis virus. J. Virol. 66: 857-864.
    
42. Piper, R.C., C. Tai, C.S. Hahn, C.M. Rice, H. Huang and D.E. James (1992) The efficient intracellular sequestration of the insulin-regulatable glucose transporter (GLUT-4) is conferred by the NH2 terminus. J. Cell Biol. 117:729-743.
    
43. Krishnan, B. R., D. Kersulyte, I. Brikun, H. V. Huang, C. M. Berg, and D. E. Berg (1993) Transposon- and PCR-based sequencing of DNAs cloned in lambda phage. Meth. Enzymol.218:258-279.
    
44. Hertz, J. and H. V. Huang. (1995) Evolution of the Sindbis virus subgenomic mRNA promoter in cultured cells. J. Virol.69: 7768-7774. (PDF)
    
45. Hertz, J. and H. V. Huang. (1995) Host-dependent evolution of the Sindbis virus promoter for subgenomic mRNA synthesis. J. Virol.69:7775-7781. (PDF)
    
46. Aker, M. and H. V. Huang. (1996). Extreme heterogeneity of centromeric minor satellite repeats in inbred strains of mice. Mammalian Genome 7:62-64 (PDF)
    
47. Frolov, I., T. A. Hoffman, B. M. Prágai, S. A. Dryga, H. V. Huang, S. Schlesinger, C. M. Rice. (1996) Alphavirus-based expression vectors: Strategies and applications. Proc. Natl. Acad. Sci. USA 93:11371-11377. (PDF)
    
48. Huang, H. V. (1996) Sindbis virus vectors for expression in animal cells. Curr. Opin. Biotechnol. 7:531-535.
    
49. Huang, H. V. (1997) Evolution of the alphavirus promoter and the cis-acting sequences of RNA viruses. in Factors in the Emergence of Arbovirus Diseases, J.-F. Saluzzo and B. Dodet (eds). Elsevier Press, Paris. pp 65-79.
    
50. Wielgosz, M., and H. V. Huang. (1997) A novel viral RNA species in Sindbis virus-infected cells. J. Virol. 71:9108-9117. (PDF)
    
51. Mouslim C., Hilbert F, Huang H, Groisman EA. (2002) Conflicting needs for a Salmonella hypervirulence gene in host and non-host environments. Mol Micro 45:1019-1027.
    
52. Hunag H. V. and S. Schlesinger (2003). Virus-based vectors for gene expression in mammalian cells: Sindbis virus. in Gene Transfer and Expression in Mammalian Cells, S. C. Makrides (ed). Elsevier Press, Amsterdam. pp189-205.
    
53. Zwir I, D. Shin, A. Kato, K. Nishino, T. Latifi, F. Solomon, J. M. Hare, H. Huang, E. A. Groisman (2005) Dissecting the PhoP regulatory network of Escherichia coli and Salmonella enterica. Proc. Natl. Acad. Sci. USA 102(8):2862-2867.
54. Zwir, I., H. Huang, E. A. Groisman (2005) Analysis of differentially-regulated genes within a regulatory network by GPS genome navigation. Bioinformatics 21:4073-4083.
55. Shin, D., E.-J. Lee, H. Huang, E. A. Groisman (2006) A positive feedback loop promotes transcription surge that jump-starts Salmonella virulence circuit. Science, in press.

Technology for Licensing:

Patents
"Automated method for quantitative analysis of biological fluids". US patent 4,327,073.
"Sindbis virus vectors". US patent 5,091,309.
"DNA transposon Tn5seq1". US patent 5,137,829.
"Method of DNA sequencing using DNA transposon Tn5seqL". US patents 5,212,080, 5,869,296.
"Infectious Sindbis virus vectors". US patent 5,217,879.

Sindbis virus gene expression vectors
These are infectious RNA virus vectors with excellent levels of gene expression in a variety of vertebrate and invertebrate cells.
They can be used as expression vectors, vaccine reagents, for non-integrating genetic immunization, etc.