Curriculum Vitae
Niraj H. Tolia


PubMed Publications: for tolia + NH
Division of Biology and Biomedical Sciences profile

Office Address:

Department of Molecular Microbiology
Campus Box 8230, 660 S. Euclid Avenue
Washington University School of Medicine
St. Louis, Missouri 63110-1093
Tel: 314-286-0134 Fax: 314-362-1232
Email: tolia [at] wustl.edu

   

 

 

Present Position:

Associate Professor, Departments of Molecular Microbiology and Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO

Education/Training:

1999 - 2004 Ph. D. Biological Sciences, Watson School of Biological Sciences, Cold Spring Harbor Laboratory, Cold Spring Harbor,  NY, USA
1995 - 1999 B.Sc. Biochemistry, Imperial College of Science, Technology and Medicine, London, UK.

Professional Experience and Appointments:

2007 - present

Departments of Molecular Microbiology and Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO, USA

2004 - 2007

Postdoctoral Fellow, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA

Honors and Awards:

2014-2019 Investigators in the Pathogenesis of Infectious Disease Award, Burroughs Wellcome Fund
2010 Grants for New Investigators, Edward Mallinckrodt, Jr. Foundation
2006 Malaria: Functional Genomics to Biology to Medicine Meeting Scholarship, Keystone Symposia
1999-2004 Leslie Quick, Jr. Fellowship, Watson School of Biological Sciences
1999-2004 C. Ewart Stickings Memorial Prize. Distinction award from the Department of Biochemistry, Imperial College
1998 IMPACT Award, Pfizer Central Research award for innovation

University Service and Committees:

2015 MESA malERA Refresh Consortium
2014 - present Secretary/Treasurer, American Committee of Molecular, Cellular and Immunoparasitology (ACMCIP), American Society of Tropical Medicine and Hygiene (ASTMH)
2014 - present Scientific Program Committee, American Society of Tropical Medicine and Hygiene (ASTMH)
2014 - present Steering Committee: Molecular Microbiology & Microbial Pathogenesis Program
2014 - present Molecular Microbiology & Microbial Pathogenesis Seminar Series Committee
2012 - 2014 Co-Chair PhD Admissions Committee, The Division of Biology and Biomedical Sciences, Washington University, St. Louis, MO
2008 - 2014 PhD Admissions Committee, The Division of Biology and Biomedical Sciences, Washington University, St. Louis, MO
2009 - 2014 Course Master for the Infectious Diseases/Basic Microbiological Mechanisms Research Conference

Teaching:

2013 - present Co-Course Master - L41 BIO 5328 - Structural Biology Journal Club
2012 - present Co-Course Master, Lecturer - Bio 5456 Advanced Crystallography
2010 - present Lecturer in Molecular Microbiology and Microbial Pathogenesis (Biol 5392)
2009 - present Lecturer in Special Topics in Microbial Pathogenesis (Biol 5217)

Publications:

    ·  Meyer PA, Socias S, Key J, Ransey E, Tjon EC, Buschiazzo A, Lei M, Botka C, Withrow J, Neau D, Rajashankar K, Anderson KS, Baxter RH, Blacklow SC, Boggon TJ, Bonvin AM, Borek D, Brett TJ, Caflisch A, Chang CI, Chazin WJ, Corbett KD, Cosgrove MS, Crosson S, Dhe-Paganon S, Di Cera E, Drennan CL, Eck MJ, Eichman BF, Fan QR, Ferré-D'Amaré AR, Christopher Fromme J, Garcia KC, Gaudet R, Gong P, Harrison SC, Heldwein EE, Jia Z, Keenan RJ, Kruse AC, Kvansakul M, McLellan JS, Modis Y, Nam Y, Otwinowski Z, Pai EF, Pereira PJ, Petosa C, Raman CS, Rapoport TA, Roll-Mecak A, Rosen MK, Rudenko G, Schlessinger J, Schwartz TU, Shamoo Y, Sondermann H, Tao YJ, Tolia NH, Tsodikov OV, Westover KD, Wu H, Foster I, Fraser JS, Maia FR, Gonen T, Kirchhausen T, Diederichs K, Crosas M, Sliz P, "Data publication with the structural biology data grid supports live analysis." Nature Communications (2016) Mar 7;7:10882.

    ·  Nichole D Salinas, Niraj H Tolia, "Red cell receptors as access points for malaria infection." Current Opinion in Hematology (2016) Jan 13.

    ·  Jooyoung Park#, Ann M Guggisberg#, Audrey R Odom*, Niraj H Tolia*, "Cap-domain closure enables diverse substrate recognition by the C2-type haloacid dehalogenase-like sugar phosphatase Plasmodium falciparum HAD1." Acta Crystallographica Section D (2015) Sep;71(Pt 9):1824-34. doi: 10.1107/S1399004715012067. # co-first author, * co-senior author

    ·  Michael S Behnke, Asis Khan , Elvin J Lauron, John R Jimah, Qiuling Wang, Niraj H Tolia, L David Sibley, "Rhoptry Proteins ROP5 and ROP18 Are Major Murine Virulence Factors in Genetically Divergent South American Strains of Toxoplasma gondii." PLOS Genetics (2015) Aug 20;11(8):e1005434. doi: 10.1371/journal.pgen.1005434

    ·  Edwin Chen, Nichole D Salinas, Francis B Ntumngia, John H Adams, Niraj H Tolia, "Structural analysis of the synthetic Duffy Binding Protein (DBP) antigen DEKnull relevant for Plasmodium vivax malaria vaccine design." PLOS Neglected Tropical Diseases (2015) Mar 20;9(3):e0003644.

    ·  Xiao L Xu, Shawei Chen, Nichole D Salinas, Niraj H Tolia, Gregory A. Grant "Comparison of Type 1 D-3-Phosphoglycerate Dehydrogenases Reveals Unique Regulation in Pathogenic Mycobacteria." Archives of Biochemistry and Biophysics (2015) Feb 16. pii: S0003-9861(15)00070-3. doi: 10.1016/j.abb.2015.02.008.

    ·  Joshua Muia, Jian Zhu, Garima Gupta, Sandra L. Haberichter, Kenneth D. Friedman, Hendrik B. Feys, Louis Deforche, Karen Vanhoorelbeke, Lisa A. Westfield, Robyn Roth, Niraj H Tolia, John E. Heuser, J. Evan Sadler. "Allosteric activation of ADAMTS13 by von Willebrand factor." Proceedings of the National Academy of Sciences (2014) Dec 15. pii: 201413282.

    ·  Nichole D Salinas#, May M Paing#, Niraj H Tolia. "Critical glycosylated residues in exon three of erythrocyte glycophorin A engage Plasmodium falciparum EBA-175 and define receptor specificity." mBio (2014) 5(5):01606-14. doi:10.1128/mBio.01606-14.

    ·  Ann M Guggisberg#, Jooyoung Park#, Rachel L Edwards, Megan L Kelly, Dana M Hodge, Niraj H Tolia*, Audrey R Odom*. "A sugar phosphatase regulates the methylerythritol phosphate (MEP) pathway in malaria parasites." Nature Communications (2014) 5:4467. doi: 10.1038/ncomms5467. # co-first author, * co-senior author

    ·  May M Paing, Niraj H Tolia. "Multimeric Assembly of Host-Pathogen Adhesion Complexes Involved in Apicomplexan Invasion" PLOS Pathogens (2014) 10(6): e1004120. doi:10.1371/journal.ppat.1004120

    ·  Brian M Malpede, Niraj H Tolia. "Malaria Adhesins: Structure and Function." Cellular Microbiology (2014) May;16(5):621-31. doi: 10.1111/cmi.12276.

    ·  Joseph D Batchelor, Brian M Malpede, Natalie S Omattage, Gregory T DeKoster, Katherine A Henzler-Wildman, Niraj H Tolia. "Red Blood Cell Invasion by Plasmodium vivax: Structural Basis for DBP Engagement of DARC." PLOS Pathogens (2014) 10(1): e1003869. doi:10.1371/journal.ppat.1003869 Pubmed Article Media Coverage: The Times of India Science Daily Eurekalert WUSTL News

    ·  Nichole D Salinas, Niraj H Tolia. "A quantitative assay for binding and inhibition of Plasmodium falciparum Erythrocyte Binding Antigen 175 reveals high affinity binding depends on both DBL domains." Protein Expression and Purification (2014) pii: S1046-5928(13)00277-5. doi: 10.1016/j.pep.2013.12.008.

    ·  Aida S. Badiane, Amy K. Bei, Ambroise D. Ahouidi, Saurabh D. Patel, Nichole Salinas, Daouda Ndiaye, Ousmane Sarr, Omar Ndir, Niraj H Tolia, Souleymane Mboup, and Manoj T. Duraisingh. "Inhibitory humoral responses to the Plasmodium falciparum vaccine candidate EBA-175 are independent of erythrocyte invasion pathway." Clinincal Vaccine Immunology (2013) 20(8):1238-45. doi: 10.1128/CVI.00135-13. Epub 2013 Jun 12.

    ·  Edwin Chen, May M. Paing, Nichole Salinas, B. Kim Lee Sim, Niraj H Tolia. "Structural and Functional Basis for Inhibition of Erythrocyte Invasion by Antibodies that Target Plasmodium falciparum EBA-175." PLOS Pathogens (2013) 9(5): e1003390. doi:10.1371/journal.ppat.1003390

    ·  Brian M Malpede, Daniel H Lin, Niraj H Tolia. "Molecular Basis for Sialic Acid-dependent Receptor Recognition by Plasmodium falciparum Erythrocyte Binding Antigen 140/BAEBL." Journal of Biological Chemistry (2013) Apr 26;288(17):12406-15. Epub Mar 18.

    ·  Daniel H Lin, Brian M Malpede, Joseph D Batchelor, Niraj H Tolia. "Crystal and Solution Structures of Plasmodium falciparum Erythrocyte Binding Antigen 140 Reveal Determinants of Receptor Specificity during Erythrocyte Invasion." Journal of Biological Chemistry (2012) Oct 26;287(44):36830-6. Epub Sep 18.

    ·  Joseph D Batchelor, Jacob A Zahm, Niraj H Tolia. "Dimerization of Plasmodium vivax DBP is induced upon receptor binding and drives recognition of DARC." Nature Structural & Molecular Biology (2011) Jul 10;18(8):908-14

    ·  Niraj H Tolia, Eric J Enemark, B Kim Lee Sim, Leemor Joshua-Tor. "Structural basis for the EBA-175 erythrocyte invasion pathway of the malaria parasite P. falciparum." Cell (2005) Jul 29; 122(2):183-93

    ·  Niraj H Tolia, Leemor Joshua-Tor. "Slicer and the Argonautes" Nature Chemical Biology (2007) Jan;3(1):36-43. Pubmed Article

    ·  Erbay Yigit, Pedro J. Batista, Yanxia Bei, Ka Ming Pang, Chun-Chieh G. Chen, Niraj H Tolia, Leemor Joshua-Tor, Shohei Mitani, Martin J. Simard, Craig C Mello. "Analysis of the C. elegans Argonaute family reveals that distinct Argonautes act sequentially during RNAi" Cell (2006) Nov 17;127(4):747-757.

    ·  Danielle V Irvine*, Mikel Zaratiegui*, Niraj H Tolia*, Derek B Goto, Daniel H Chitwood, Matthew W Vaughn, Leemor Joshua-Tor, Robert A Martienssen "Argonaute slicing is required for heterochromatic silencing and spreading." Science (2006) Aug 25; 313(5790):1134-7. Pubmed Article *co-first authors

    ·  Niraj H Tolia*, Leemor Joshua-Tor. "Strategies for protein coexpression in Escherichia coli." Nature Methods (2006) Jan; 3(1):55-64 Pubmed Article *corresponding author

    ·  Fabiola V Rivas*, Niraj H Tolia*, Ji-Joon Song*, Juan P Aragon, Jidong Liu, Gregory J Hannon, Leemor Joshua-Tor. "Purified Argonaute2 and an siRNA form recombinant human RISC." Nature Structural & Molecular Biology (2005) Apr; 12(4):340-9. Epub Mar 30. *co-first authors

    ·  Ji-Joon Song, Jidong Liu, Niraj H Tolia, Jonathan Schneiderman, Stephanie K Smith, Robert A Martienssen, Gregory J Hannon, Leemor Joshua-Tor. "The crystal structure of the Argonaute2 PAZ domain reveals an RNA binding motif in RNAi effector complexes." Nature Structural Biology (2003) Dec; 10(12):1026-32. Epub Nov 16.