Associate Professor of Molecular Microbiology
RNA virus evolution. Molecular biology of alphaviruses. Alphavirus gene expression vectors. Antiviral drug design.
Department of Molecular Microbiology
Washington University School of Medicine
8230 McDonnell Pediatric Research Building
Campus Box 8230
660 S. Euclid Ave.
St. Louis, MO 63110
Phone, office: 314-362-2755
1992 – Associate Professor Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO
1972 A.B. Biology, Occidental College, Los Angeles, California
1977 Ph.D. Biochemistry, California Institute of Technology, Pasadena, California. Thesis advisor: Dr. William J. Dreyer.
Professional Experience and Appointments:
1982-1992 Assistant Professor, Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO
1981-1982 Senior Research Fellow, Division of Biology, California Institute of Technology, with Dr. Leroy Hood
1982 Invited Instructor, faculty course on techniques in molecular biology, California State University, Northridge, California
1980-1981 Senior Research Fellow in Biology and Biomedical Engineering, Divisions of Biology, and Engineering and Applied Science, California Institute of Technology, with Drs. Leroy Hood and Derek Fender
1979-1980 Research Fellow in Biology and Biomedical Engineering, Divisions of Biology, and Engineering and Applied Science, California Institute of Technology, with Drs. Leroy Hood and Derek Fender
1979-1980 Part time Instructor, Division of Biology, California Institute of Technology
1977-1979 American Cancer Society Postdoctoral Fellow, Division of Biology, California Institute of Technology, with Dr. Leroy Hood
1971-1972 Member of BUGSYS design team for computer analysis of video images, Jet Propulsion Laboratory, California Institute of Technology
1970 Seltzer Award, Occidental College
1970-1972 College Scholar, Occidental College
1972 A.B. Summa Cum Laude, Occidental College
1972 Alpha Mu Gamma, National Honorary Language Fraternity1972Phi Beta Kappa
1972 Association for the Advancement of Biomedical Education Award
1972-1977 Danforth Fellow
1976-1977 McCallum Fellowship, California Institute of Technology
1977-1979 American Cancer Society Postdoctoral Fellow
1995-1999 Member, NIH Virology Study Section
1997-present International Committee on Taxonomy of Viruses. Togaviridae Study Group
1982- Ad Hoc Reviewer: Journal of Virology, Proceedings of the National Academy of Science, USA, Virus Research, Nucleic Acids Research
Professional Society Memberships:
American Association for the Advancement of ScienceAmerican Society for MicrobiologyAmerican Society for VirologyGenetics Society of AmericaSigma Xi
University Appointments and Committees:
Marion E. Andrew
Charles F. Austerberry
Frederick G. Barr
Jenny M. Buzan
Anthony M. Dean
Karen W. Dodson
Brooke HarmonJennifer Hertz*
William C. Hollifield*
Thomas M. J. Niederman
J Christian Perez
Scott R. SchaecherMichael V. Seiden
Judy A. C. Thomas
Ruth W. Yokoyama
* Thesis advisor of these students
1983-5 Member, Nucleic Acids Preliminary Examination Committee
1986 Chair, Nucleic Acids Preliminary Examination Committee
1985-86, 88 Member, Developmental Biology Preliminary Examination Committee
1987 Chair, Developmental Biology Preliminary Examination Committee
1988 Member, Genetics Preliminary Examination Committees
1991 Chair, Virology Preliminary Examination Committee
1983 Member, Cell and Molecular Biology Programs Admissions Committee
1984-87 Secretary, Cell and Molecular Biology Programs Admissions Committee
1988 Secretary, Cell, Immunology and Molecular Biology Programs Admissions Committee
1988-1992 Member, Medical Scientist Training Program Committee
1991 Medical Scientist Training Program Director Search Committee
1990-present Supervisor, Departmental computing services
1986-1988 Member, Steering Committee of the Molecular Biology: Genetics and Biochemistry Program
1988-1991 Co-coordinator, Molecular Biology: Genetics and Biochemistry Program
1991-1992 Coordinator, Molecular Microbiology & Microbial Pathogenesis Program
1991-1992 Member, Governing Committee of the Programs in Cell and Molecular Biology
1990-1991 Senior Advisory Committee1991Co-chairman, Cell Molecular Biology Merger Committee
1992 Member, Programs and Student Affairs Committee
1992-1994 Member, Minority subcommittee, Committee on Admissions, School of Medicine
1992-1996 Member, Recruitment Committee of the Division of Biology and Biomedical Sciences
1997 Information Services Committee, of the Medical School Process Improvement Program
1996-2000 Member, Committee on Admissions, School of Medicine
2001-2005 Member, Institutional Biological and Chemical Safety Committee2005-presentCo-chair, Institutional Biological and Chemical Safety Committee
1983 Microbiology: Molecular Biology of Prokaryotes
1983, 1984 Foundations in Immunology1983, 1984Topics in Immunology
1983, 1986 Regulatory Phenomena in Cell and Molecular Biology
1984 Selected Topics in Developmental Biology: Mechanisms of DNA Recombination and Rearrangement
1987 Advanced Genetics
1989 Practical Applications of Viruses1990-91Microbiology1994Molecular Virology1995Ethics and Research Science
1995-1998 Co-coursemaster, Molecular Virology
1997-present Molecular Microbiology and Pathogenesis
1999-2004 Coursemaster, Molecular Virology
1999-2001 Microbes and Pathogenesis
2000-2001 Discussion group leader, Nucleic acids and Protein Synthesis
2004 Co-coursemaster, Special Topics in Microbial Pathogenesis
2002-present Co-coursemaster, Microbes and Pathogenesis
Participation on Training Grants:
1984-present Cell and Molecular Biology Training Grant
1984-present Program Physician-Scientist Award
1984-present Infectious Disease Training Grant
Peer Review Panels:
1982-present Ad hoc and special reviewer / site visit panels, for NIADDK, NIAID, NSF.
1995-1999Member, Virology study section, NIH.
1989-1997 Judge, Monsanto-St. Louis Post Dispatch Science Fair, Honors Division.
1994-2000 Technology Committee, Forsyth School
1995-2003 Board of Trustees, Forsyth School
Huang, H.V., R.S. Molday and W.J. Dreyer (1973). Isoelectric focusing of rod outer segment membrane proteins. FEBS Letters 37: 285-289.
Lim, R.W., R.S. Molday, H.V. Huang and S.-P.S. Yen (1975). Application of latex microspheres in the isolation of plasma membranes. Affinity density perturbation of erythrocyte membranes. Biochim. Biophys. Acta 394: 377-387.
Hood, L., H.V. Huang and W.J. Dreyer (1977). The Area Code Hypothesis: The immune system provides clues to understanding the genetic and molecular basis of cell recognition during development. J. Supramolec. Struct. 7: 531-559.
Huang, H.V. and W.J. Dreyer (1978). Bursectomy in ovo blocks the generation of immunoglobulin diversity. J. Immunol. 121: 1738-1747.
Hood, J.M., H.V. Huang and L. Hood (1980). A computer simulation of evolutionary forces controlling the size of a multigene family. J. Mol. Evol. 15: 181-197.
Early, P., H. Huang, M. Davis, K. Calame and L. Hood (1980). An immunoglobulin heavy chain variable region gene is generated from three segments of DNA: VH, D and JH. Cell 19: 981-992.
Huang, H.V., S.T. Crews and L. Hood (1981). The arrangement, rearrangement and diversification of antibody genes. In Frontiers in Immunogenetics, W. Hildemann and J. Frelinger (eds), Elsevier-North Holland, New York. pp63-74.
Huang, H.V., S.T. Crews and L. Hood (1981). Diversification of antibody genes through DNA rearrangement. Adv Exp Med Biol 137:475-488.
Crews, S.T., J. Griffin, H. Huang, K. Calame and L. Hood (1981). A single VH gene segment encodes target=”ref” the immune response to phosphocholine: Somatic mutation is correlated with the class of the antibody. Cell 25: 59-66.
Huang, H.V., S.T. Crews and L. Hood (1981). An immunoglobulin VH pseudogene. J. Appl. Molec. Genet. 1: 93-101.
Hood, L., M. Davis, P. Early, K. Calame, S. Kim, S. Crews and H. Huang (1981). Two types of DNA rearrangements in immunoglobulin genes. Cold Spring Harbor Symp. Quant. Biol. 45: 887-898.
Hood, L. J. Griffin, S. Crews, H. Huang, M. Kronenberg and S. Kim (1981). Antibody and MHC genes. In Immunoglobulin Idiotypes and their Expression. C. Janeway, E. Sercarz, H. Wigzell and C. Fred Fox (eds), Academic Press, New York. ICN-UCLA Symp. Molec. Cell. Biol. 20: 805-824.
Huang, H.V. and L. Hood (1982). The expression of antibody genes. In Comparative Research on Leukemia and Related Diseases. D.S. Yohn and J.R. Blakeslee (eds), Elsevier-North Holland, New York. pp 169-172.
Hunkapiller, T., H. Huang, L. Hood and J. Campbell (1982). The impact of modern genetics on evolutionary theory. In Perspectives on Evolution. R. Milkman (ed), Sinauer Assoc., Sunderland, Mass. pp 164-189.
Hood, L., J. Griffin, S. Crews, H. Huang, K. Kronenberg and S. Kim (1982). Antibody and MHC genes. Oncodev Biol. Med. 4: 61-79.
Huang, H.V., M. Bond, M. Hunkapiller and L. Hood (1983). Efficient cleavage of proteins at tryptophanyl residues using DMSO-HCl and CNBr. Meth. Enzymol. 91: 318-324.
Huang, H.V. and L. Hood (1983). The ontogenetic and evolutionary origin of V gene formation. Stadler Genetics Symp. Proc. 14: 59-68.
Huang, H.V. and L. Hood (1983). Origins of antibody diversity. In Recombinant DNA and Medical Genetics. L. Porter (ed). Academic Press, New York. Birth Defects Symp. 13: 97-104.
Levis, R., B.G. Weiss, M. Tsiang, H. Huang and S. Schlesinger (1986). Deletion mapping of Sindbis virus DI RNAs derived from cDNAs defines the sequences essential for replication and packaging. Cell 44: 137-145.
Shen, P. and H.V. Huang (1986). Homologous recombination in Escherichia coli: Dependence on substrate length and homology. Genetics 112: 441-457.
Lutz, C.T., T.L. Bartholow, N.S. Greenspan, R.J. Fulton, W.J. Monafo, R.M. Perlmutter, H.V. Huang and J.M. Davie (1987). Molecular dissection of the murine antibody response to streptococcal group A carbohydrate. J. Exp. Med. 165: 531-545.
Schlesinger, S., R. Levis, B.G. Weiss, M. Tsiang and H. Huang (1987). Replication and packaging sequences in defective interfering RNAs of Sindbis virus. In Positive Strand RNA Viruses, M. Brinton and R. Rueckert (eds).
Alan R. Liss, New York. pp 241-250.
Siu, G., E. A. Springer, H. V. Huang, L. E. Hood and S. T. Crews (1987). Structure of the T15 VH gene subfamily: Identification of immunoglobulin gene promotor homologies. J. Immunol. 138: 4466-4471.
Lutz, C.T., W.C. Hollifield, B. Seed, J.M. Davie and H.V. Huang (1987). Syrinx2A: An improved lambda phage vector designed for screening DNA libraries by recombination in vivo. Proc. Natl. Acad. Sci. USA 84: 4379-4383.
Levis, R., S. Schlesinger and H.V. Huang (1987). Engineered defective interfering RNAs of Sindbis virus express bacterial chloramphenicol acetyltransferase in avian cells. Proc. Natl. Acad. Sci. USA 84: 4811-4815.
Rice, C.M., R. Levis, J.H. Strauss and H.V. Huang (1987). Production of infectious RNA transcripts from Sindbis virus cDNA clones: Mapping of lethal mutations, rescue of a temperature-sensitive marker, and in vitro mutagenesis to generate defined mutants. J. Virol. 61:3809-3819.
Hollifield, W.C., E.N. Kaplan and H.V. Huang (1987). Efficient RecABC-dependent, homologous recombination between lambda and plasmids requires a phage ninR region gene. Molec. Gen. Genet.210:248-255.
Nag, D. K., H. V. Huang and D. E. Berg (1988). Bidirectional chain terminating DNA sequencing strategy using transposon Tn5-seq as a source of mobile primer sites. Gene 64:135-145.
Polo, J. M., N. L. Davis, C. M. Rice, H. V. Huang and R. E. Johnston. (1988) Molecular analysis of Sindbis virus pathogenesis in neonatal mice using virus recombinants constructed in vitro. J. Virol. 62:2124-2133.
Huang, H.V., P. Little and B. Seed (1988). Improved suppressor tRNA cloning vectors and plasmid-phage recombination. Biotechnology 10:269-83
Schlesinger, S., C. Xiong, R. Levis, C. M. Rice and H. V. Huang. (1988) Development of Sindbis virus and defective interfering RNAs as expression vectors. In Viral Vectors, Y. Gluzman and S. H. Hughes (eds). Cold
Spring Harbor Banbury Conference Report. Cold Spring Harbor Laboratory, New York. pp 167-171.
Xiong, C., R. Levis, P. Shen, S. Schlesinger, C. M. Rice and H. V. Huang. (1989) Sindbis virus: an efficient, broad host range vector for gene expression in animal cells. Science 243:1188-1191.
Phadnis, S., H. V. Huang and D. E. Berg. (1989) Tn5supF, a 264 bp transposon derived from Tn5 for insertion mutagenesis and sequencing DNAs cloned in phage lambda. Proc. Natl. Acad. Sci. USA 86:5908-5912.
Shen, P. and H.V. Huang. (1989) Effect of base pair mismatches on recombination via the RecBCD pathway. Molec. Gen. Genet. 218:358-360.
Mi, S., Durbin, R., Huang, H. V., Rice, C. M., and Stollar, V. (1989). Association of the Sindbis virus RNA methyltransferase activity with the nonstructural protein nsP1. Virology. 170:385-391.
Grakoui, A., R. Levis, R. Raju, H. V. Huang and C. M. Rice. (1989) A cis-acting mutation in the Sindbis virus junction region affecting subgenomic RNA synthesis. J. Virol. 63:5216-5227.
Huang, H. V., C. M. Rice, C. Xiong and S. Schlesinger. (1989) RNA Viruses as Gene Expression Vectors. Virus Genes 3: 85-91.
Grakoui, A., R. Levis, R. Raju, S. Schlesinger, H. V. Huang and C. M. Rice. (1990) Cis-acting sequence responsible for Sindbis virus subgenomic RNA synthesis. in New Aspects of Positive-Strand RNA Viruses, M. A. Brinton & F. X. Heinz (ed). American Society of Microbiology, Washington D.C. pp108-115.
Levis, R., S. Schlesinger and H. V. Huang. (1990) The promoter for Sindbis virus RNA-dependent subgenomic mRNA transcription. J. Virol. 64:1726-1733.
Raju, R. and H. V. Huang. (1991) Analysis of Sindbis virus promoter recognition in vivo using novel vectors with two subgenomic mRNA promoters. J. Virol. 65:2501-2510.
Hertz, J. and H. V. Huang. (1992) Utilization of heterologous alphavirus junction sequences as promoters by Sindbis virus. J. Virol. 66: 857-864.
Piper, R.C., C. Tai, C.S. Hahn, C.M. Rice, H. Huang and D.E. James (1992) The efficient intracellular sequestration of the insulin-regulatable glucose transporter (GLUT-4) is conferred by the NH2 terminus. J. Cell Biol. 117:729-743.
Krishnan, B. R., D. Kersulyte, I. Brikun, H. V. Huang, C. M. Berg, and D. E. Berg (1993) Transposon- and PCR-based sequencing of DNAs cloned in lambda phage. Meth. Enzymol.218:258-279.
Frolov, I., T. A. Hoffman, B. M. Prágai, S. A. Dryga, H. V. Huang, S. Schlesinger, C. M. Rice. (1996) Alphavirus-based expression vectors: Strategies and applications. Proc. Natl. Acad. Sci. USA 93:11371-11377.(PDF)
Huang, H. V. (1996) Sindbis virus vectors for expression in animal cells. Curr. Opin. Biotechnol. 7:531-535.
Huang, H. V. (1997) Evolution of the alphavirus promoter and the cis-acting sequences of RNA viruses. in Factors in the Emergence of Arbovirus Diseases, J.-F. Saluzzo and B. Dodet (eds). Elsevier Press, Paris. pp 65-79.
Mouslim C., Hilbert F, Huang H, Groisman EA. (2002) Conflicting needs for a Salmonella hypervirulence gene in host and non-host environments. Mol Micro 45:1019-1027.
Hunag H. V. and S. Schlesinger (2003). Virus-based vectors for gene expression in mammalian cells: Sindbis virus. in Gene Transfer and Expression in Mammalian Cells, S. C. Makrides (ed). Elsevier Press, Amsterdam. pp189-205.
Zwir I, D. Shin, A. Kato, K. Nishino, T. Latifi, F. Solomon, J. M. Hare, H. Huang, E. A. Groisman (2005) Dissecting the PhoP regulatory network of Escherichia coli and Salmonella enterica. Proc. Natl. Acad. Sci. USA 102(8):2862-2867.
Zwir, I., H. Huang, E. A. Groisman (2005) Analysis of differentially-regulated genes within a regulatory network by GPS genome navigation. Bioinformatics 21:4073-4083.
Shin, D., E.-J. Lee, H. Huang, E. A. Groisman (2006) A positive feedback loop promotes transcription surge that jump-starts Salmonella virulence circuit. Science, in press.
Technology for Licensing: Patents
“Automated method for quantitative analysis of biological fluids”. US patent 4,327,073.
“Sindbis virus vectors”. US patent 5,091,309.
“DNA transposon Tn5seq1”. US patent 5,137,829.
“Method of DNA sequencing using DNA transposon Tn5seqL”. US patents 5,212,080, 5,869,296.
“Infectious Sindbis virus vectors”. US patent 5,217,879. Sindbis virus gene expression vectors
These are infectious RNA virus vectors with excellent levels of gene expression in a variety of vertebrate and invertebrate cells.
They can be used as expression vectors, vaccine reagents, for non-integrating genetic immunization, etc.