Professor of Molecular Microbiology

Caparon Lab website

Research Interests:

Pathogenesis of infections caused by Gram positive bacteria. Gene regulation and mechanisms of toxin secretion by Group A streptococci.

PubMed publications: for Caparon + MG
Division of Biology and Biomedical Sciences profile

Office address:

Departments of Molecular Microbiology and Pathology & Immunology
Washington University School of Medicine
10260 McDonnell Pediatric Research Building
Campus Box 8230
660 S. Euclid Ave.
St. Louis, MO 63110

Phone, office: 314-362-1485
Fax: 314-362-1232
Email: caparon [at] wusm.wustl.edu

Education:

1981 B.S. Michigan State University, Microbiology
1985 Ph.D. University of Iowa, Microbiology
1989 Emory University (Post-doc), Microbiology

Current Position:

Professor, Department of Molecular Microbiology, Washington University, St. Louis, MO, 2002-present

Academic positions & employment:

1981-1985 Graduate Assistant, Department of Microbiology
1985-1987 Postdoctoral Trainee, Emory University, with Dr. June R. Scott
1987-1988 Associate, Department of Microbiology and Immunology, Emory University
1988-1989 Senior Associate, Department of Microbiology and Immunology, Emory University
1989-1997 Assistant Professor, Department of Molecular Microbiology, Washington University
1997-2002 Associate Professor, Department of Molecular Microbiology, Washington University
2002-present Professor, Department of Molecular Microbiology, Washington University

Selected Peer Reviewed Publications:

Caparon, M. G. and Scott, J. R. (1987). Identification of a gene that regulates expression of M.protein, the major virulence determinant of group A streptococci. Proc. Natl. Acad. Sci. USA 84:8677-8681. PMID: 2446327.
Scott, J. R., Kirchman, P. A., Caparon, M. G. (1988). An intermediate in transposition of the conjugative transposon Tn916. Proc. Natl. Acad. USA Sci 85:4809-4813. PMID: 2838847.

Caparon, M. G. and Scott, J. R. (1989). Excision and Insertion of the conjugative transposon Tn916 involves a novel recombination mechanism. Cell. 59: 1027-1034. PMID: 2557157.

Hanski, E. and Caparon, M. G. (1992). Protein F, a fibronectin-binding protein, is an adhesin of the group A streptococcus. Proc. Natl. Acad. Sci. USA. 89:6172-6176. PMID: 1385871.

Okada, N., Geist, R. T., and Caparon, M. G. (1993). Positive transcriptional control of mry regulates virulence in the group A streptococcus. Mol. Microbiol. 7:893-903. PMID: 8483419.

Van Heyningen, T., G. Fogg, D. Yates, E. Hanski, and M. G. Caparon. 1993. Adherence and fibronectin-binding are environmentally regulated in the group A streptococcus. Mol. Microbiol. 7:893-903. PMID: 7934934.

Geist, R. T., N. Okada, and M. G. Caparon. 1993. Analysis of Streptococcus pyogenes promoters using Novel Tn916-based shuttle vectors for the construction of transcriptional fusions to CAT. J. Bacteriol. 175:7561-7570. PMID: 8244925.

Fogg, G., C. Gibson, and M. G. Caparon. 1994. The identification of rofA, a positive-acting regulatory component of prtF expression: Use of a mgd-based shuttle mutagenesis strategy in Streptococcus pyogenes. Mol. Microbiol. 11:671-684. PMID: 8196542.

Okada, N., A. P. Pentland, P. Falk and M. G. Caparon. 1994. M protein and protein F act as Important Determinants of Cell-Specific Tropism of Streptococcus pyogenes in Skin Tissue. J. Clin. Invest. 94:965-977. PMID: 8083381.

Okada, N., M. K. Liszewski, J. Atkinson and M. Caparon. 1995. Membrane co-factor protein (MCP or CD46) is a keratinocyte receptor for the M protein of Streptococcus pyogenes. Proc. Natl. Acad. Sci. USA. 92:2489-2493. PMID: 7708671.

Ozeri, V., S. Natanson-Yaron, M. G. Caparon, K. M. Yamada, S. K. Akiyama, I. Vlodavsky and E. Hanski. 1996. A two-domain mechanism for group A streptococcal adherence through protein F to the extracellular matrix. EMBO J. 15:989-998. PMID: 8605894.

Gibson, C. M., and M. G. Caparon. 1996. Insertional inactivation of Streptococcus pyogenes sod suggests prtF is regulated in response to a superoxide signal. J. Bacteriol. 178:4688-4695. PMID: 8755901.

Wang, B., N. Ruiz, A. Pentland, and M. Caparon. 1997. Keratinocyte proinflammaroy responses to adherent and non-adherent group A streptococci. Infect. Immun. 65:2119-2126. PMID: 9169741.

Fogg, G. C., and M. G. Caparon. 1997. Constitutive expression of fibronectin-binding in Streptococcus pyogenes as a result of anaerobic activation of rofA. J. Bacteriol. 179:6172-6180. PMID: 9324268.

Ruiz, N., B. Wang, A. Pentland, and M. Caparon. 1997. Streptolysin O and adherence synergistically modulate proinflammatory responses of keratinocytes to group A strep. Mol. Microbiol. 27:337-346. PMID: 9484889.

Lyon, W., C. Gibson, and M. Caparon. 1998. A role for Trigger Factor and an Rgg-like regulator in the transcription, secretion and processing of the cysteine proteinase of Streptococcus pyogenes. EMBO J. 21:6263-6275. PMID: 9799235.

Gibson, C. M., T. C. Mallett, A. Claiborne, and M. G. Caparon. 2000. The contribution of NADH oxidase to the aerobic metabolism of Streptococcus pyogenes. J. Bacteriol. 182: 448-455. PMID: 10629192.

Granok, A., D. Parsonage, R. P. Ross, and M. G. Caparon. 2000. The RofA binding site of Streptococcus pyogenes is utilized by multiple transcriptional pathways. J. Bacteriol. 182:1529-1540. PMID: 10692357.

King, K., J. Hornstein, and M. Caparon. 2000. Aerotolerance and peroxide resistance in peroxidase and PerR mutants of Streptococcus pyogenes. J. Bacteriol. 182:5290-5299. PMID: 10986229.

Lyon, W., J. C. Madden, and M. G. Caparon. 2001. Mutation of LuxS affects growth and virulence factor expression in Streptococcus pyogenes. Mol. Microbiol. 42:145-157. PMID: 11679074.

Francis, K. P., J. Yu, C. Bellinger-Kawahara, D. Joh, M. J. Hawkinson, G. Xiao, T. F. Purchio, M. G. Caparon, M. Lipsitch, and P. R. Contag. 2001. Viusalizing pneumococcal infections int he lungs of live mice using bioluminescent Streptococcus pneumoniae transformed with a novel Gram-positive lux transposon. Infect. Immun. 69:3350-3358. PMID: 11292758.

Madden, J. C., N. R. Ruiz, and M. Caparon. 2001. Cytolysin-mediated Translocation (CMT): A functional equivalent of type III secretion for Gram-positive bacteria. Cell. 104:143-152. PMID: 11163247.

Gibson, C. M. and M. Caparon. .2002. .An alkaline phophatase reporter transposon for identification of genes encoding secreted proteins in Gram-positive organisms. App. Environ. Microbiol. 68:928-932. PMID: 11823238.

Neely, M. N., Pfeifer, J. D., and M. Caparon. 2002. Streptococcus-Zebrafish model of bacterial pathogenesis. Infect. Immun. 70:3904-3914. PMID: 12065534.

Lyon, W. and M. G. Caparon. 2003. Trigger factor-mediated prolyl isomerization influences maturation of the Streptococcus pyogenes cysteine protease. J. Bacteriol. 185:3661-3667. PMID: 14977969.

Neely, M. N., W. R. Lyon, D. L. Runft and M. Caparon. 2003. Role of RopB in growth phase expression of the SpeB cysteine protease of Streptococcus pyogenes. J Bacteriol. 185::5166-5174. PMID: 12923089.

Brenot, A., K. Y. King, B. Janowiak, O. Griffith and M. Caparon. 2004. Contribution of glutathione peroxidase to the virulence of Streptococcus pyogenes. Infect. Immun. 72:408-413. PMID: 14688122.

Lyon, W. and M. Caparon. 2004. A role for the serine protease HtrA (DegP) of Streptococcus pyogenes in the biogenesis of the virulence factors SpeB and SLS. Infect. Immun. 72:1618-1625.

Nyberg, P. T. Sakai K. H. Cho, M. G. Caparon, R. Fassler, L. Björck. 2004. Interactions with fibronectin attenuate the virulence of Streptococcus pyogenes. EMBO J. 23:2166-2174. PMID: 15103329.

Meehl, M. and M. Caparon. 2004. Specificity of streptolysin O in cytolysin-mediated translocation. Mol. Microbiol. 52:1665-1676. PMID: 15186416.

Rosch, J. W. and M. Caparon. 2004. A microdomain for protein secretion in Gram-positive bacteria. Science. 304:1513-1515. PMID: 15178803.

Kau, A., M., S. Martin, M. Caparon*, and S. Hultgren* 2004. A novel murine model demonstrates Enterococcus faecalis tropism for mouse kidney. Infect. Immun. 73:2164-2168. PMID: 15784592.

Brenot, A., K. Y. King and M. Caparon. 2004. The role of the PerR regulon in peroxide resistance and virulence of Streptococcus pyogenes. Mol. Microbiol. 55:221-234. PMID: 15612930.

Cho, K. H. and M. G. Caparon. 2005. Patterns of virulence gene expression differ between biofilm and tissue communities of Streptococcus pyogenes. Mol. Microbiol. 57:1545-1556.. PMID: 16135223.

Meehl, M. A., J. S. Pinkner, P. J. Anderson. C. Smith, S. J. Hultgren and M. Caparon. 2005. Immunity factor for SPN (IFS): An endogenous inhibitor of the secreted NAD-glycohydrolase of Streptococcus pyogenes. PLOS Pathogens. 1:e35. PMID: 16333395.

Rosch, J. and M. Caparon. 2005. The ExPortal: An organelle dedicated to the biogenesis of secreted proteins in Streptococcus pyogenes. Mol. Microbiol. . 58:959-968.. PMID: 16262783.

Loughman, J. A. and M. Caparon. 2006. Regulation of SpeB in Streptococcus pyogenes by pH and NaCl: A model for in vivo gene expression. J. Bacteriol. 188:398-408.. PMID: 16385029.

Ghosh, J. and M Caparon. 2006. Specificity of Streptococcus pyogenes NAD glycohydrolase in cytolysin-mediated translocation. Mol. Microbiol. 64:1203-1214. PMID: 17042787.

Loughman, J. A., and M. G. Caparon. 2006. A novel adaptation of aldolase regulates virulence in Streptococcus pyogenes. EMBO J. 25:5414-5422. PMID: 17066081.

Loughman, J. A. and M. G. Caparon. 2006. Contribution of invariant residues to the function of Rgg family transcription regulators. J. Bacteriol. 189:650-655. PMID: 17098902.

Rosch, J., F. F. Hsu, and M. Caparon. 2007. Anionic phospholipids enriched at the ExPortal of Streptococcus pyogenes. J. Bacteriol. 189-801-806. PMID: 17142392.

Brenot, A., B. F. Weston, and M. G. Caparon. 2007. A PerR-regulated metal transporter (PmtA) is an interface between oxidative stress and metal homeostasis in Streptococcus pyogenes. Mol. Microbiol. 63:1185-1196. PMID: 17238923.

Loughman, J. A. and M. G. Caparon. 2007. Comparative functional analysis of the lac operons in Streptococcus pyogenes. Mol. Microbiol. 264:269-280. PMID: 17371500.

Cole, J., J. A. Aquilina, P. G. Hains, A. Henningham, K. S. Sriprakash, M. G. Caparon, V. Nizet, M. Kotb, S. J. Cordwell, S. P. Djordjevic, M. J. Walker. 2007. Role of group A Streptococcus HtrA in the maturation of the SpeB protease. Proteomics. 7:4488-4498. PMID: 18072207.

Cho, K. H. and M. G. Caparon. 2008. tRNA modification by GidA/MnmE is necessary for Streptococcus pyogenes virulence: A new strategy to make live attenuated strains. Infect. Immun. 76:3176-3186.. PMID: 18426891.

Rosch, J. W., L. A. Vega, J. Beyer, A. Lin and M. G. Caparon. 2008. The signal recognition particle pathway is required for virulence in Streptococcus pyogenes. Infect. Immun. 76:2612-2619. PMID: 18411293.